A comparison of binocular and monocular surgery on the effect of eye-covering pretreatment on emergence delirium after general anesthesia in pre-school aged children / 中国实用护理杂志

Objective:To evaluate the effect of eye-covering pretreatment on acute delirium in ophthalmology preschool-age children who underwent binocular and monocular surgery by general anesthesia.Methods:The 300 preschool-age children who underwent general anesthesia of elective ophthalmic surgery in the Second Affiliated Hospital, Zhejiang University School of Medicine, from August 2019 to February 2021 were selected as the research object. They were divided into control group and blindfold group with 150 cases each by random number-table. Children in the control group received regular education on cartoon animation videos before surgery; children in the blindfold group received eye-covering pretreatment on the basis of cartoon animation videos(monocular surgery with monocular cover, binocular surgery with binocular cover). The Modified Yale Preoperative Anxiety Scale (m-YPAS) , the Nursing Delirium Screening Scale(NU-DESC), the incidence rate of delirium and the score of postoperative nursing difficulty were compared between two groups.Results:The 271 cases were completed in this study, including 129 cases(monocular surgery 66 cases, binocular surgery 63 cases) in the blindfold group and 142 cases (monocular surgery 73 cases, binocular surgery 69 cases) in the control group. The preoperative m-YPAS score, the postoperative NU-DESC score, the incidence rate of acute delirium and postoperative nursing care difficulty score of monocular surgery in the blindfold group , monocular surgery was (40.28 ± 15.02) points, 1.00 (0.00, 2.00) points, 27.3%(18/66), 1.00 (1.00, 2.00) points,and binocular surgery was (41.69 ± 16.35) points, 1.00 (0.00, 2.00), 39.7%(25/63), 1.00(1.00, 2.00); in the control group, monocular surgery was (46.28 ± 15.76) points, 2.00 (1.00, 3.00) points, 67.1% (49/73), 2.00 (1.00, 3.00) points, and binocular surgery was (47.77 ± 14.82) points, 3.00 (2.00, 4.00) points, 82.6% (57/69) and 2.00 (1.50, 3.00) points respectively. The difference between the two groups was statistically significant ( t= -2.29, -2.24, Z values were -5.74 - -2.95, χ2= 32.94, 25.78, all P<0.05). The preoperative m-YPAS score, the postoperative NU-DESC score, the incidence rate of acute delirium and postoperative nursing care difficulty score of monocular surgery patients in the blindfold group had no significantly statistical difference with that of binocular surgery patient (all P>0.05) . Conclusions:Monocular/ binocular eye-covering pretreatment can effectively decrease the preoperative m-YPAS score, the postoperative NU-DESC score, incidence rate of acute delirium and the postoperative nursing care difficulty in preschool-age children who underwent general anesthesia both monocular or binocular surgery. There was no difference in the application effect of monocular or binocular surgery.

Endocorona, un enfoque diferente en rehabilitación oral

RESUMEN Introducción: Con el avance de la tecnología adhesiva, nuevas técnicas y materiales restauradores están siendo utilizados para el tratamiento de dientes endodonciados, por ello, las endocoronas son una alternativa clínica para su tratamiento. La ventaja de esta restauración es que tiene mejores características macro retentivas, requiere menor tiempo clínico y de laboratorio. Objetivo: Proporcionar información bibliográfica actual sobre esta nueva alternativa para aplicar una nueva guía en rehabilitación oral con el uso de la endocorona mediante una revisión de la literatura. Método: Se realizó una revisión bibliográfica detallada, con la consideración de artículos originales o casos clínicos que incluían información relacionada a las restauraciones de tipo endocorona. La búsqueda se efectuó en las bases de datos PubMed, SciELO, Science Direct, Elsevier y Google Académico. Resultados: Las restauraciones de tipo endocorona son un monobloque único que devuelve la anatomía completa de la corona dental, la cual aprovecha la cámara pulpar para así lograr una mayor adhesión y macro retención mecánica. Este enfoque restaurador proporciona una función adecuada y estética, así como la integridad biomecánica de las estructuras no vitales. Conclusiones: Actualmente en la Odontología moderna se emplea el término de mínima intervención, conservando en su mayor cantidad posible el tejido dental de manera que los dientes tengan mayor funcionalidad, por ello las endocoronas son una opción segura y con buen pronóstico a largo plazo en rehabilitación oral para tratar piezas endodonciadas, debido a que es un procedimiento técnicamente más conservador, estético, sencillo y de menor costo.

ABSTRACT Introduction: With the advancement of adhesive technology, new techniques and restorative materials are being used for the treatment of root canals; therefore endocrowns are a clinical alternative for their treatment. The advantage of this restoration is that it has better macro retentive characteristics, requires less clinical and laboratory time. Objective: To provide current bibliographic information on this new alternative to apply a new guide in oral rehabilitation with the use of endocrown through a literature review. Method: A detailed bibliographic review was carried out, with the consideration of original articles or clinical cases that included information related to endocrown-type restorations. The search was carried out in the databases PubMed, SciELO, Science Direct, Elsevier and Google Scholar. Results: Endocrown restorations are a single monoblock that rebuilds the complete anatomy of the dental crown, which takes advantage of the pulp chamber to achieve greater adhesion and macro mechanical retention. This restorative approach provides adequate function and aesthetics, as well as the biomechanical integrity of non-vital structures. Conclusions: The term minimal intervention is currently very used in modern dentistry, preserving the dental tissue as much as possible so that the teeth have greater functionality. Endocrowns are a safe option with a good long-term prognosis in oral rehabilitation to treat root canals, because it is a technically more conservative, aesthetic, simple and lower-cost procedure.

RESUMO Introdução: Com o avanço da tecnologia adesiva, novas técnicas e materiais restauradores estão sendo utilizados para o tratamento de dentes endodônticos, portanto os endocrowns são uma alternativa clínica para seu tratamento. A vantagem dessa restauração é que ela apresenta melhores características macro retentivas, requer menos tempo clínico e laboratorial. Objetivo: Fornecer informações bibliográficas atuais sobre esta nova alternativa de aplicação de um novo guia na reabilitação oral com o uso de endocrown por meio de uma revisão da literatura. Método: Foi realizada uma revisão bibliográfica detalhada, considerando artigos originais ou casos clínicos que incluíam informações relacionadas a restaurações do tipo endocrown. A busca foi realizada nas bases de dados PubMed, SciELO, Science Direct, Elsevier e Google Scholar. Resultados: As restaurações do tipo endocrown são um único monobloco que devolve a anatomia completa da coroa dentária, que aproveita a câmara pulpar para obter maior adesão e retenção macromecânica. Esta abordagem restauradora fornece função e estética adequadas, bem como a integridade biomecânica de estruturas não vitais. Conclusões: Atualmente na odontologia moderna é utilizado o termo de intervenção mínima, preservando-se o tecido dentário o máximo possível para que os dentes tenham maior funcionalidade, portanto endocrowns são uma opção segura e com bom prognóstico em longo prazo na reabilitação oral para tratamento da endodontia dentes, por ser um procedimento tecnicamente mais conservador, estético, simples e de menor custo.

Role of covering Ileostomy in causation of anastomotic strictures in low anterior resections for rectum carcinoma / O papel da ileostomia de proteção como causa das estenoses anastomóticas nas ressecções anteriores de reto

Abstract Background Colorectal resection anastomosis is the commonest cause of rectal strictures. Anastomotic site ischemia, incomplete doughnuts from stapled anastomosis and pelvic infection, are some of the risk factors that play a role in the development of postoperative rectal strictures. However, the role of diverting stoma in the development of rectal strictures has not been studied extensively. Objectives To study the difference in the occurrence of anastomotic strictures (AS) in patients submitted to low anterior resection (LAR) with covering ileostomy (CI), and to LAR without CI for carcinoma rectum. Methods This was a prospective, comparative case control study carried out at a tertiary care referral center. Low anterior resection with covering ileostomy was performed in patients with rectum carcinoma in the study group, while LAR without covering ileostomy was performed in the control group. The study group had 29 patients, while the control group had 33 patients with rectum carcinoma. Results During themean follow-up period of 9.1months, 8 (28%) patients in the study group and 2 (6%) patients in the control group developed AS (p =0.019). Out of these 8 patients with AS in the study group, 50% had Grade-I AS, 25% had Grade-II AS, while 25% of the patients had Grade-III (severe) AS. However, both patients who developed AS in the control group had a mild type (Grade I) of AS. Conclusion Covering ileostomy increases the chances of AS formation after LAR for rectum carcinoma. Also, the SKIMS Clinical Grading of Rectal Strictures is a simple and

Resumo Introdução A anastomose de ressecção colorretal é a causa mais comum de estenoses retais. A isquemia do local da anastomose, donuts (anéis) incompletos de anastomose grampeada e infecção pélvica são alguns dos fatores de risco que desempenham um papel no desenvolvimento de estenoses retais pós-operatórias. No entanto, o papel do estoma de desvio no desenvolvimento de estenoses retais não foi estudado extensivamente. Objetivos Estudar a diferença na ocorrência de estenoses anastomóticas (EA) em pacientes submetidos à ressecção anterior baixa (LAR) com ileostomia de proteção e a LAR sem ileostomia de proteção para carcinoma de reto. Métodos Este foi um estudo prospectivo e comparativo de caso-controle realizado em um centro de referência de atenção terciária. A ressecção anterior baixa com ileostomia de proteção foi realizada em pacientes com carcinoma de reto no grupo de estudo, enquanto LAR sem ileostomia de proteção foi realizada no grupo controle. O grupo de estudo tinha 29 pacientes, enquanto o grupo controle tinha 33 pacientes com carcinoma de reto. Resultados Durante o período de acompanhamento médio de 9, 1 meses, 8 (28%) pacientes no grupo de estudo e 2 (6%) pacientes no grupo controle desenvolveram EA (p=0,019). Destes 8 pacientes com EA no grupo de estudo, 50% tinham EA de Grau I, 25% tinhamEA de Grau II, enquanto 25% dos pacientes tinham EA de Grau III (grave). No entanto, ambos os pacientes que desenvolveram EA no grupo de controle tinham um tipo leve (Grau I) de EA. Conclusão A ileostomia de proteção aumenta as chances de formação de AS após LAR para carcinoma de reto. Além disso, o SKIMS Clinical Grading of Rectal Strictures é uma ferramenta simples e útil disponível para cada cirurgião para graduar, classificar e monitorar as estenoses retais pós-operatórias.

Observation of acupoint thread-embedding on refractive amblyopia in children / 中国针灸

OBJECTIVE@#To observe the effect of acupoint thread@*METHODS@#A total of 60 children with refractive amblyopia were randomized into an observation group (30 cases, 2 cases dropped off) and a control group (30 cases, 1 case dropped off). In the control group, comprehensive therapy of eye covering of intact side and family refined performance was adopted. On the basis of the treatment in the control group, acupoint thread@*RESULTS@#After treatment, the corrected vision was increased compared before treatment in the both groups (@*CONCLUSION@#Acupoint thread

Análisis del cumplimiento del plan de consumo de los medicamentos de alto costo. Matanzas. 2012-2017 / Analysis of the fulfillment of the consumption plan of high cost medications. Matanzas. 2012 - 2017

RESUMEN Introducción: los medicamentos de alto costo son medicamentos nuevos, altamente específicos y utilizados en condiciones clínicas complejas, como el tratamiento de algunos tipos de cáncer; enfermedades que comprometen el sistema inmunológico, enfermedades inflamatorias o infecciosas. Objetivo: determinar cumplimiento del plan de consumo de los medicamentos de alto costo en la provincia de Matanzas, en el quinquenio 2012-2017. Materiales y métodos: estudio descriptivo, observacional de seguimiento sobre el cumplimiento del plan de consumo de los medicamentos de alto costo, en la población matancera del año 2012 al 2017. Se analizó el universo de medicamentos incluidos en esta categoría, a partir de la base de datos de suministro de medicamentos que emplea la Empresa Comercializadora de Medicamentos. Se identificaron las variables estudiadas. Resultados: en todo el período de estudio se observó un incremento creciente de los medicamentos de alto costo, en el 88,9 % de ellos el consumo ha estado por encima de la planificación realizada por la provincia. Los costos se incrementaron en un 233 % y además en las prescripciones realizadas de estos productos, se encontraron problemas como escaques vacíos, antibióticos sin impresión diagnóstica y omisión de la forma de presentación del medicamento y/o dosis indicada. Conclusiones: el consumo de muchos medicamentos de alto costo fue mayor que la planificación realizada en la provincia de Matanzas, durante el período analizado. Implicó un incremento significativo del presupuesto destinado a estos fines y se detectaron dificultades en el cumplimiento de lo establecido en las prescripciones de dichos medicamentos (AU).

ABSTRACT Introduction: high cost medications (HCM) are new highly specific medications and used in complex clinical conditions as in treatment of some types of cancer, diseases that compromise the immunological system, inflammatory or infections disorders. Objective: to determine the fulfillment of the consumption plan of high-cost medications in the province of Matanzas in the period 2012-2017. Materials and methods: a descriptive, observational, follow up study on the fulfillment of the consumption plan of high-cost medication by the population of Matanzas2012 year to 2017. The universe of drugs included in this category was analyzed on the bases of the drug-supplying database used by the Drug Commercializing Enterprise (ENCOMED in Spanish). The studied variables were identified. Results: it was observed a growing increase of high-cost drugs use during all the period; in 88.9 % of them the consumption has been above the planning made in the province. The costs increased in 233 %, and besides that in the prescriptions made of these drugs there were found problems like empty boxes, antibiotics without diagnostic impression and omissions of the drug presentation forms and/or the prescribed doses. Conclusions: the consumption of many high-cost drugs was higher than the planning made in the province of Matanzas for the analyzed period. It implied a significant increase of the budget destined for these aims and difficulties were found in the fulfillment of the terms for prescribing these drugs (AU).

Development of Helicoverpa armigera (Hübner, 1805) and Spodoptera frugiperda (SMITH, 1797) in winter forages / Desenvolvimento de Helicoverpa armigera (Hübner, 1805) e Spodoptera frugiperda (SMITH, 1797) em forragens de inverno

Helicoverpa armigera Hübner, 1805 and Spodoptera frugiperda Smith, 1797 are polyphagous pests of great agricultural importance in subtropical and temperate climate regions. The usual management of production areas in the southern region of Brazil occurs after the harvesting of summer crops, and the areas are sown with pasture. Thus, forages recommended for grazing are azevém (Lolium multiflorumLam.) and cornichão (Lotus corniculatus L.) due to their nutritional benefits, good palatability, regrowth, and hardiness. Considering the high degree of polyphagia of H. armigera and S. frugiperda, and the impact of maintaining continuous feeding areas (green bridges) in the management of these species, this work aimed to evaluate the development of H. armigera and S. frugiperda fed leaves of azevém and cornichão under laboratory conditions. The insects were collected in the city of Capão do Leão/RS in corn and soybean plantations. For each forage species, 130 newly hatched caterpillars were each placed in autoclaved glass tubes, with one-third of the tube length containing forage. The tubes were capped with waterproof cotton and placed in a climate-controlled room at 25 ± 1°C, 70 ± 10% relative humidity, and a 12-h photophase. The food was replenished daily until the caterpillars entered the pupae stage. The pupae were sexed and weighed, and the newly emerged adults were placed in pairs in PVC cages, lined with white A4 sulfite paper. The papers used as a laying substrate were removed and changed daily, and eggs were counted. The number of instars was determined by the linearized Dyar rule model. The complete randomization design was employed for the variables biological cycle length and viability of egg, caterpillar, pre-pupa, pupa, adult, and pre-oviposition phases and weight of caterpillars on the 14th day and pupae after 24 h. Based on the results obtained, a fertility life table was prepared. H. armigera did not complete the cycle, with only three instars and a duration of 22.1 and 24.6 days in azevém and cornichão, respectively. When evaluated in S. frugiperda caterpillars, development in forage species was observed, with five and six instars and duration of 51.7 and 45.1 days in azevém and cornichão, respectively. The azevém was distinguished by interference in the development cycle of the species, reducing the effect of the green bridge. In addition, surviving insects were susceptible to the effects of the agroecosystem due to the low-quality food source.

Helicoverpa armigera Hübner, 1805 e Spodoptera frugiperda Smith, 1797 são pragas polífagas de grande importância agrícola, em regiões de clima subtropical e temperado. Após a colheita das culturas de verão no sul do Brasil, as áreas são utilizadas como pastagem. Com isso, as forrageiras recomendadas ao pastoreio são cornichão (Lotuscorniculatus L.) e azevém (Loliummultiflorum Lam.) por apresentarem benefícios nutricional, palatabilidade, rebrota e rusticidade. Devido ao alto grau de polifagia de H. armigera e S. frugiperda e o impacto de áreas continuas de alimento (pontes verdes) no manejo destas espécies, este trabalho objetivou-se avaliar o desenvolvimento de H. armigera e S. frugiperda alimentadas com azevém e cornichão em condições de laboratório. Os insetos foram coletados no município de Capão do Leão/RS em plantios de milho e soja. Para cada espécie forrageira foram individualizadas 130 lagartas recém-eclodidas em tubos de vidro autoclavados, com um terço (1/3) do seu comprimento contendo a respectiva forrageira. Os tubos foram tampados com algodão hidrófugo e levados para a sala climatizada a 25 ± 1°C, UR de 70 ± 10% e 12 horas de fotofase. Diariamente, o alimento foi reposto até que as lagartas entrarem em estágio de pupa. As pupas foram sexadas e pesadas e os adultos recém-emergidos foram individualizados em casais em gaiolas de PVC, forradas com papel sulfite A4 branco no seu interior. Os papéis utilizados como substrato de postura foram retirados e trocados diariamente e os ovos foram contabilizados. O número de instares foi determinado pelo modelo linearizado da regra de Dyar. O delineamento utilizado foi inteiramente casualizado (DIC) para as variáveis duração do ciclo biológico e viabilidade das fases de ovo, lagarta, pré-pupa, pupa, adultos e pré-oviposição, peso de lagartas ao decimo quarto (14°) dia e pupas após 24 horas. Com base nos resultados obtidos, foi elaborada tabela de vida de fertilidade. H. armigera não completou o ciclo, com apenas três instares e duração de 22,1 e 24,6 dias em azevém e cornichão, respectivamente. Quando avaliado em lagartas de S. frugiperda observou o desenvolvimento nas espécies forrageiras, com 5 e 6 instares e duração de 51,7 e 45,1 dias em azevém e cornichão, respectivamente. Contudo, considerando o critério que evite a formação de ponte verde, azevém destacou-se por interferir no ciclo de desenvolvimento das espécies. Onde, mesmos os que sobrevivem, estão suscetíveis aos efeitos do agroecossitema devido a fonte alimentar ser de baixa qualidade.

Esculent coating of spider silk enhanced the preservation and shelf life of apricot / A cobertura escamada de seda de aranha aumentou a preservação e a vida útil do damasco

Abstract According to the Food and Agriculture Organization (FAO), roughly one-third of the total food produced is lost globally. The major cause of this wastage is the perishability of fruits and vegetables. Therefore, researchers have endeavored to develop an effective preservation technique. Our study explored the potential application of spider silk as an odourless and edible preservative coating for fruits. The spider silk was collected from spiders reared in the laboratory, following by degumming and dissolution to formulate the silk solution. For this study, apricots were selected as the model fruit. The apricots were dip coated with the formulated silk solution and allowed to dry. In order to enhance the beta sheet content of the silk coating, the fruits were exposed to water annealing for varying intervals of time under vacuum condition. The effect of silk coating and water annealing time period on preservation of fruits was then evaluated morphologically and gravimetrically. The results showed that the fruits, which were used as control, exhibited a greater degree of water loss and suffered from fungal attack. In contrast, the silk coated fruits showed less water loss and were protected from fungal attack. Therefore, the study provides compelling evidence regarding the application of spider silk as a preservative coating.

Resumo Segundo a Organização para Alimentação e Agricultura (FAO), cerca de um terço do total de alimentos produzidos é perdido globalmente. A principal causa deste desperdício é a perecibilidade das frutas e legumes. Portanto, os pesquisadores têm se esforçado para desenvolver uma técnica de preservação eficaz. Nosso estudo explorou a potencial aplicação de seda de aranha como um revestimento conservante inodoro e comestível para frutas. A seda da aranha foi coletada de aranhas criadas em laboratório, seguindo-se a degomagem e dissolução para formular a solução de seda. Para este estudo, os damascos foram selecionados como fruto modelo. Os damascos foram revestidos por imersão com a solução de seda formulada e deixados secar. A fim de aumentar o teor de folhas beta do revestimento de seda, os frutos foram expostos a recozimento de água durante vários intervalos de tempo sob condições de vácuo. O efeito do tempo de recobrimento de seda e recozimento de água na preservação de frutos foi então avaliado morfologicamente e gravimetricamente. Os resultados mostraram que os frutos, utilizados como controle, apresentaram maior grau de perda de água e sofreram ataque fúngico. Em contraste, os frutos revestidos de seda mostraram menos perda de água e foram protegidos contra ataques fúngicos. Portanto, o estudo fornece evidências convincentes sobre a aplicação de seda de aranha como um revestimento conservante.

Evaluation of postoperative patient satisfaction after covering the nasal dorsum with upper lateral cartilage: "upper lateral closing" / Avaliação da satisfação pós-operatória do paciente após cobertura do dorso nasal com a cartilagem lateral superior: "fechamento lateral superior"

Abstract Introduction: Following nasal hump removal during septorhinoplasty, the middle vault should be reconstructed to avoid functional and esthetic problems. Middle vault reconstruction, however, may result in widening of the middle vault and may need a camouflage graft to cover dorsal irregularities. Objective: To present the results of reconstructing the middle vault with a technique that covers the nasal dorsum with upper lateral cartilage, from the viewpoint of patient satisfaction. Methods: Retrospective study of patients who underwent septorhinoplasty that included nasal dorsum closure with upper lateral cartilage from December 1, 2014 to January 31, 2016. Those with postoperative follow-up of less than 3 months were excluded. The final study group included 39 patients. The same surgeon performed all septorhinoplasties. The dorsum was closed using an "upper lateral closing" technique that approximated upper lateral cartilages to each other over the septum. Postoperative patient satisfaction was determined using a visual analog scale and the rhinoplasty outcomes evaluation questionnaire. The questionnaire evaluates patient esthetic and functional satisfaction with the operated nose. High scores indicate improved esthetic results. Results: No dorsal irregularities were seen at postoperative follow-up evaluation of the patients. For esthetic nasal appearance, the median visual analogue scale scores was 86%, and the mean for the questionnaire was 77.03%. Conclusion: The natural dome-shaped anatomy of the nasal dorsum was achieved by approximating the upper lateral cartilages to each other. Closing the dorsum with this technique also covers any dorsal irregularities and results in a smooth dorsum. Patients expressed satisfaction with the esthetic and functional aspects of the smooth, attractive nasal dorsum.

Resumo Introdução: Após a remoção da giba nasal durante a rinosseptoplastia, a abóbada deve ser reconstruída para evitar problemas funcionais e estéticos. A reconstrução da abóboda entretanto, pode resultar em alargamento dorsal e pode necessitar de um enxerto para camuflar irregularidades dorsais. Objetivo: Avaliar a satisfação dos pacientes com os resultados da reconstrução da abóboda com uma técnica que utiliza a cartilagem lateral superior para recobrir o dorso nasal. Método: Estudo retrospectivo de pacientes submetidos a rinosseptoplastia, que incluiu fechamento do dorso nasal com cartilagem lateral superior, realizado de 1º de dezembro de 2014 a 31 de janeiro de 2016. Foram excluídos aqueles com acompanhamento pós-operatório de menos de 3 meses. O grupo final do estudo incluiu 39 pacientes. O mesmo cirurgião realizou todas as rinosseptoplastias. O dorso foi fechado com uma técnica de "fechamento lateral superior" que aproxima as cartilagens laterais superiores de cada lado sobre o septo. A satisfação pós-operatória dos pacientes foi determinada através de uma escala visual analógica e o questionário Rhinoplasty Outcomes Evaluation. O questionário avalia a satisfação estética e funcional do paciente com o nariz. Escores altos indicam percepção de melhoria estética. Resultados: Não foram observadas irregularidades dorsais na avaliação pós-operatória de seguimento dos pacientes. Em relação à aparência nasal estética, o escore médio da escala visual analógica foi 86% e o escore médio do questionário foi 77,03%. Conclusões: A anatomia natural em forma de domo do dorso nasal foi conseguida através da aproximação das cartilagens laterais superiores entre si. O fechamento do dorso com essa técnica também abrange todas as irregularidades dorsais e resulta em um dorso liso. Os pacientes expressaram satisfação com os aspectos estéticos e funcionais do dorso nasal liso e atraente.

Clinical observation of autologous penetrating keratoplasty in the treatment of corneal blindness / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the clinical effect of autologous penetrating keratoplasty in the treatment of corneal blindness. <p>METHODS: Totally 8 patients with corneal blindness were treated by autologous penetrating keratoplasty in our hospital from 2014-01 to 2018-03. Using retrospective analysis, the patients were followed up for one year. To observe the intraoperative complications and postoperative conditions such as visual acuity, corneal transparency, and other were observed.<p>RESULTS: The uncorrected visual acuity of all the 8 patients was greater than 0.02 1wk after operation, and the rate of restoration of visual acuity was 100%(8/8). The corrected visual acuity of 5 patients(5-8)was more than 0.3 1mo after operation. The corrected visual acuity of 3 patients(3-8)with severe cataract before operation was improved to 0.08-0.2. One year later, all the corneal grafts in the recipient eyes were transparency and no recurrence of infection or secondary infection occurred in all 8 patients. <p>CONCLUSION: Corneal graft is easy to grow and there are no exclusion reactions, fewer postoperative complications after autologous corneal transplantation. So, corneal implants can remain transparent for a long time, and the surgery cost is lower. Autologous corneal transplantation can not only provide long-term useful vision for patients with monocular blindness combined with corneal blindness, but also reduce their financial burden and bring great benefits to patients.

Study on quality standard of imitation ecological planting Anoectochilus roxburghii under forest covering / 中草药

Objective To study the quality standard of the imitation ecological planting Anoectochilus roxburghii under forest covering. Methods The identification of medicinal properties, microscopic characteristics, thin-layer chromatography (TLC) and content determination of A. roxburghii cultivated under forest were carried out in this study, and the moisture content, ash and acid-insoluble ash were determined according to Chinese Pharmacopoeia of 2015 edition. Results A. roxburghii cultivated under forest exhibits specific properties in characteristics, microscopic features and TLC results. The average moisture content of A. roxburghii cultivated under forest from five planting bases was 8.69%, the average ash was 11.93%, and the average acid-insoluble ash was 3.27%. Content determination results of the average quality score of quercetin, isorhamnetin and kaempferide were at 0.021 0%,,0.024 7%, and 0.027 3%, respectively. Conclusion The above method is simple, specific and reproducible, which will provide the basis for the quality standard of the imitation ecological planting A. roxburghii under forest covering.

Palliative surgery for refractory purulent corneal ulcer / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM:To investigate the clinical effect of scleral allograft combined with conjunctival flap autograft for refractory purulent corneal ulcer. <p>METHODS: Twenty patients(20 eyes)with purulent corneal ulcer admitted to our hospital during June 2015 to June 2017 were selected. The results showed that the drug treatment was not effective and could not be performed by penetrating keratoplasty, and then allogeneic scleral transplantation combined with conjunctival flap autograft covering was performed. A retrospective analysis was carried out. The intraoperative complications, postoperative scleral and conjunctival flap growth and postoperative complications of this palliative operation were observed. <p>RESULTS: Of the 20 patients, 18 had good growth of sclera and conjunctival flap, the cure rate was 90%. One case had bad healing of conjunctival flap and was cured by amniotic membrane transplantation; 1 case had enucleation because of severe vitreous cavity infection; 2 cases had lens prolapse, the incidence rate was 10%. Secondary glaucoma occurred in 3 cases with an incidence of 15%, which was cured by cryociliary surgery.<p>CONCLUSION:Scleral allograft combined with conjunctival flap autograft is a palliative operation. It is an effective way to control the corneal infection and maintain the appearance of the eyeball in the patients with severe purulent corneal ulcer, which is ineffective in drug treatment and unable to perform penetrating keratoplasty. It can replace the previous enucleation of the eye contents to control the infection. It can not only avoid the eye pain and psychological trauma caused by the loss of eyeball, but also save money for improving the appearance in the later stage.

Clinical effect of levodopa and benserazide hydrochloride tables combined with visual occlusion for amblyopia in children / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM:To study the therapeutic effect of visual occlusion combined with levodopa and benserazide hydrochloride tables on children with amblyopia. <p>METHODS: Totally 90 cases(140 eyes)of children diagnosed with amblyopia in our hospital were selected from January 2016 to January 2017. They were randomly divided into the monotherapy group and the combined treatment group, and 35 healthy children(70 eyes)were selected as the normal group for comparison. Patients in the monotherapy group were treated with visual cover, while patients in the combined treatment group were treated with oral administration of levodopa and benserazide tablets on the basis of visual cover. Tears were extracted from both groups of children before and after treatment, and the protein levels of CREB and PKA in tears of 140 eyes and 70 eyes of children in the normal group were detected by enzyme-linked immunosorbent assay. The index levels of the two groups and the normal group were compared, as well as the therapeutic efficiency of different age groups and the total therapeutic efficiency of different methods. <p>RESULTS: After treatment, the levels of IL-1β, IL-6 and IL-9 in the combined treatment group were significantly lower than those in the single treatment group after treatment(<i>P</i><0.05).After treatment, the levels of CREB and PKA in the combined treatment group were significantly lower than those in the single treatment group(<i>P</i><0.05).The total effective rate of children at the age of 3-6 in the combined treatment group and the single treatment group was significantly higher than those at the age of 7-9 and 10-12 in each group(<i>P</i><0.05). The total therapeutic efficiency of the combined treatment group was significantly higher than that of the single treatment group, and the difference was statistically significant(<i>P</i><0.05). <p>CONCLUSION: Combined with the traditional masking method, levodopa and benserazide hydrochloride tables can improve the treatment of children's amblyopia. The earlier the treatment time, the better and the higher efficiency is.

Comparison between internal limiting membrane covering and internal limiting membrane peeling for giant macular hole / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the effects of par plana vitrectomy(PPV)+ inner limiting membrane(ILM)flat covering + vitreous cavity disinfected air filling and PPV + ILM stripping + vitreous cavity disinfected air filling on giant idiopathic macular hole(IMH)and high myopia macular hole(MH). <p>METHODS: The clinical data of giant IMH 40 eyes and giant high myopia MH 40 eyes were compared. Twenty patients with giant IMH underwent traditional ILM removal(Group A1), the remaining 20 underwent ILM flat transplantation(Group A2); 20 underwent traditional ILM removal(Group B1)for giant high myopic MH, and the remaining 20 underwent ILM flat transplantation(Group B2). The closure rate of MH and the improvement of best corrected visual acuity(BCVA)before and after operation were compared and analyzed. <p>RESULTS: There were significant differences in BCVA before and after operation in Group A(<i>F</i>=96.193, <i>P</i><0.001), between A1 and A2(<i>F</i>=4.971, <i>P</i>=0.03), and the interaction between different time points and groups after operation(<i>F</i>=18.772, <i>P</i><0.001). The results showed that there were significant differences of the two groups between any two time point(<i>P</i><0.05). The <i>t</i>-test results between the two groups at different time showed that there was no difference in preoperative vision between A1 and A2(<i>P</i>>0.05). There were significant differences in preoperative visual acuity between the two groups at 1, 3 and 6mo after operation(<i>P</i><0.05). There were significant differences in different time points before and after BCVA in Group B(<i>F</i>=136.150, <i>P</i><0.001), B1 and B2(<i>F</i>=5.179, <i>P</i>=0.029), and the interaction between different time points and groups after BCVA(<i>F</i>=7.079, <i>P</i>=0.001). The results showed that there were significant differences of the two groups between any two time point(<i>P</i><0.05). The results of <i>t</i>-test between the two groups at different time showed that there was no difference in preoperative visual acuity between B1 and B2(<i>P</i>>0.05), but there was significant difference in 1, 3 and 6mo after operation(<i>P</i><0.05). After 6-month follow-up, the closure rate of Group A1 was 80%, attached rate was 20%, closure rate of Group A2 was 100%. There was no significant difference in closure rate between Group A1 and Group A2(<i>P</i>=0.053). The closure rate of Group B1 was 70%, attached rate was 30%, closure rate of Group B2 was 90%, attached rate was 10%. There was no significant difference in closure rate between Group B1 and Group B2(<i>P</i>=0.118). There was significant difference in closure rate between retinal initial membrane stripping group and plaster group(75% <i>vs</i> 95%, <i>χ²</i>=4.057, <i>P</i><0.05).<p>CONCLUSION: For giant IMH and giant high myopia MH, there was significant difference in closure rate and BCVA improvement between ILM stripping group and covering group, on which the former is better.

Esculent coating of spider silk enhanced the preservation and shelf life of apricot

Abstract According to the Food and Agriculture Organization (FAO), roughly one-third of the total food produced is lost globally. The major cause of this wastage is the perishability of fruits and vegetables. Therefore, researchers have endeavored to develop an effective preservation technique. Our study explored the potential application of spider silk as an odourless and edible preservative coating for fruits. The spider silk was collected from spiders reared in the laboratory, following by degumming and dissolution to formulate the silk solution. For this study, apricots were selected as the model fruit. The apricots were dip coated with the formulated silk solution and allowed to dry. In order to enhance the beta sheet content of the silk coating, the fruits were exposed to water annealing for varying intervals of time under vacuum condition. The effect of silk coating and water annealing time period on preservation of fruits was then evaluated morphologically and gravimetrically. The results showed that the fruits, which were used as control, exhibited a greater degree of water loss and suffered from fungal attack. In contrast, the silk coated fruits showed less water loss and were protected from fungal attack. Therefore, the study provides compelling evidence regarding the application of spider silk as a preservative coating.

Resumo Segundo a Organização para Alimentação e Agricultura (FAO), cerca de um terço do total de alimentos produzidos é perdido globalmente. A principal causa deste desperdício é a perecibilidade das frutas e legumes. Portanto, os pesquisadores têm se esforçado para desenvolver uma técnica de preservação eficaz. Nosso estudo explorou a potencial aplicação de seda de aranha como um revestimento conservante inodoro e comestível para frutas. A seda da aranha foi coletada de aranhas criadas em laboratório, seguindo-se a degomagem e dissolução para formular a solução de seda. Para este estudo, os damascos foram selecionados como fruto modelo. Os damascos foram revestidos por imersão com a solução de seda formulada e deixados secar. A fim de aumentar o teor de folhas beta do revestimento de seda, os frutos foram expostos a recozimento de água durante vários intervalos de tempo sob condições de vácuo. O efeito do tempo de recobrimento de seda e recozimento de água na preservação de frutos foi então avaliado morfologicamente e gravimetricamente. Os resultados mostraram que os frutos, utilizados como controle, apresentaram maior grau de perda de água e sofreram ataque fúngico. Em contraste, os frutos revestidos de seda mostraram menos perda de água e foram protegidos contra ataques fúngicos. Portanto, o estudo fornece evidências convincentes sobre a aplicação de seda de aranha como um revestimento conservante.

Therapeutic effects of conjunctival flap covering combined with subconjunctival injection of fluconazole on patients with severe fungal corneal ulcer / 国际眼科杂志(Guoji Yanke Zazhi)

AIM:To analyze the clinical efficacy of conjunctival flap covering combined with subconjunctival injection of fluconazole on patients with severe fungal corneal ulcer.METHODS:Totally 50 cases with severe fungal corneal ulcer were collected in our hospital from January 2012 to December 2015.The cases were randomly divided into observation group and control group,25 cases in each group.All the cases were monocular sick.The control group took single drug treatment,but the observation group were given with conjunctival flap as an extra.The clinical efficacy,best corrected visual acuity and adverse effects rate were observed.And,the relapse of the two groups was followed for 1a.RESULTS:After 1 mo of treatment,the clinical curative effect of the observation group was significantly higher than the control group (P＜ 0.05),and corrected visual acuity of the observation group was higher than the control group (P＜0.05);the adverse reaction rate of the observation group was lower than the control group (P＜0.05).After 1a follow-up,the observation group only had 1 case of recurrence,control group had 8 cases with recurrence,the recurrence rate of the observation group was significantly lower than the control group (P＜0.05).CONCLUSION:The conjunctival flap combined with subconjunctival injection of fluconazole has good application value in severe fungal corneal ulcer patients,can effectively improve the clinical curative effect and the visual acuity,and can effectively reduce the incidence of adverse reactions and recurrence rate.

Research on the clinical efficacy and safety of atropine with short covering for amblyopia in children / 国际眼科杂志(Guoji Yanke Zazhi)

Abstract?AIM: To discuss clinical efficacy and safety of 10g/L atropine with short covering for children with amblyopia.?METHODS: Eighty -eight children ( 88 eyes ) with amblyopia, staying in hospital from February 2011 to February 2014 for treatment, were divided into control group ( n =44 ) and observation group ( n =44 ) . The control group only given short covering therapy was observed.Observation group was given 10g/L atropine treatment besides covering.Clinical efficacy, treatment compliance, visual acuity, corrected spherical degree of amblyopia eye and adverse events were observed and compared.?RESULTS:1) After treatment, total effective rate of the observation group was 95% ( 42/44 ) , significantly higher than that of control group ( 80%, 35/44, P0.05), but increased number of lines of vision and corrected spherical degree of amblyopia eye in the observation group were significantly higher (P<0.05);4) in the observation group total rate of adverse events was 9% ( 4/44 ) , significantly lower than that in the control group (23%, 10/44, P<0.05).?CONCLUSION: The combined therapy, 1% atropine with short covering, is effective and safe for amblyopia in children.

Actividad antifúngica del aceite esencial de naranja (Citrus sinensis L.) sobre hongos postcosecha en frutos de lechosa (Carica papaya L.) / Anti-fungal activity of essential orange oil (Citrus sinensis L.) over post-harvest fungi in papaya fruits (Carica papaya L.)

Los hongos postcosecha causan pudriciones que afectan la calidad de los frutos de lechosa (Carica papaya L.). Actualmente los aceites esenciales son considerados una alternativa a los fungicidas químicos para controlar estos hongos. En este estudio se evaluó la actividad antifúngica del aceite esencial de naranja (Citrus sinensis L.) sobre los hongos Colletotrichum gloeosporioides, Penicillium indicum, Fusarium solani, Rhizopus stolonifer y Aspergillus flavus, en medio de cultivo Papa Dextrosa Agar (PDA) en un diseño completamente al azar. Los resultados mostraron que existe un efecto inhibidor del aceite esencial de naranja (AEN) en el crecimiento micelial de los hongos in vitro, superior al 80% a concentración de AE de 1%, y 100% de inhibición a concentraciones de 2,5% y 5% de AE (p<0,05). Al ser utilizado como recubrimiento de los frutos, a concentraciones de 2,5% y 5%, disminuye la presencia de lesiones en los mismos, sin diferencias significativas (p<0,05), con igual comportamiento en efectividad in vitro e in vivo. El AEN puede ser una alternativa factible, para el control natural y eficaz de hongos postcosecha, causantes de grandes pérdidas en frutas para exportación y consumo.

Post-harvest fungi produce rotting that affects the quality of papaya fruits (Carica papaya L.) At present, essential oils are considered an alternative to chemical anti-fungal substances for controlling these fungi. In this study we evaluated the antifungal activity of essential orange oil (Citrus sinensis L.) over Colletotrichum gloeosporioides, Penicillum indicum, Fusarium solani, Rhizopus stolonifer and Aspergillus flavus, in Potato Dextrose Agar (PDA) culture medium in a completely random design. The results showed that there is an inhibitory effect of essential orange oil (EOO) over the in vitro mycelium growth of fungi, higher than 80% at a 1% EOO concentration and 100% inhibition at 2.5% and 5% EOO concentrations (p<0.05). When used as covering over the fruits at 2.5% and 5% concentrations, it decreased the presence of lesions without significant differences (p<0.05), with similar effectiveness in vitro and in vivo. EOO can be a feasible alternative for the natural and effective control of post-harvest fungi, which cause great losses in exportation and consumption fruits.

Carcaças e componentes não-carcaça de cordeiros terminados em pasto de azevém recebendo suplementação concentrada / Carcasses and non carcasses components of lambs on Italian ryegrass receiving concentrate supplementation

O trabalho foi desenvolvido com o objetivo de avaliar a resposta das características objetivas e subjetivas das carcaças e dos não componentes da carcaça de cordeiros desmamados precocemente e terminados em pasto de azevém anual ao fornecimento crescente de suplemento concentrado (sem suplementação; 0,9%; 1,8% do peso corporal e ad libitum) na dieta. Os cordeiros foram abatidos ao atingirem 32kg de peso corporal e medidas corporais foram tomadas antes do abate. Após o abate, registraram-se os pesos da carcaça quente, do conteúdo digestivo e dos não-componentes da carcaça. As carcaças permaneceram por 24 horas em câmara fria a 5°C, obtendo-se o peso da carcaça fria. Obtiveram-se os rendimentos e as medidas objetivas e subjetivas das carcaças e, por cálculos, as compacidades de perna e carcaça. Os dados foram submetidos às análises de regressão e de correlação simples. A suplementação concentrada apresentou efeito linear positivo (P<0,05) sobre pesos e rendimentos de carcaça quente e fria, compacidade de perna e de carcaça, estado de engorduramento e conformação das carcaças. A oferta crescente de suplementação concentrada aos cordeiros jovens desmamados e terminados em pasto de azevém promoveu a obtenção de carcaças maiores e com rendimento superior, com melhor conformação e estado de engorduramento, podendo resultar em melhores oportunidades de comercialização e de preços pagos ao produtor.

The study was performed to evaluate the subjective and objective measurements of the carcasses and non-carcasses components of weaned lambs grazing on Italian ryegrass pasture fed with different levels of concentrate supplementation (without supplementation, 0,9%, 1,8% of body weight and ad libitum). Lambs were slaughtered at 32kg of live weight. After slaughtering, hot carcass, digestive content and weight of non-carcass components were recorded. Weight of cold carcasses was assessed after the refrigeration for 24h at 5°C. Weight, yield and carcasses measurements were taken and then the leg and carcass compactness were calculated. Data were subjected to regression and simple correlation analysis. The concentrate supplementation levels had a positive linear effect on the weights and yields of hot and cold carcass and leg and carcasses compactness. Supplementation of weaned lambs grazing on annual ryegrass enabled to get carcasses of better conformation and fat covering. These effects may result in better price opportunities and marketing for farmers.

Associação entre o ângulo de Norberg, o percentual de cobertura da cabeça femoral, o índice cortical e o ângulo de inclinação em cães com displasia coxofemoral / Associations among Norberg angle, percentage of femoral head coverage, cortico-medullary index, and femoral inclination angle in dogs with hip dysplasia

Foram avaliadas 386 radiografias da articulação coxofemoral, sendo 220 de cães da raça Pastor Alemão, 112 machos e 108 fêmeas, e 166 da raça Labrador Retriever, 69 machos e 97 fêmeas. As radiografias foram classificadas segundo o grau de displasia coxofemoral (DCF), e foram mensurados o ângulo de inclinação, o ângulo de Norberg, o índice cortical e o percentual de cobertura da cabeça femoral de ambos os lados. As variáveis foram associadas mediante análise estatística multivariada de componentes principais. As variáveis índice cortical e ângulo de inclinação foram inversamente associadas. A raça Pastor Alemão apresentou valores de índice cortical e graus de DCF mais baixos em relação à raça Labrador Retriever. Maior ângulo de inclinação foi associado a menor ângulo de Norberg e menor percentual de cobertura. Animais mais velhos apresentaram menor ângulo de Norberg, menor porcentagem de cobertura e maior grau de DCF. Nas fêmeas, foram observados menor porcentagem de cobertura, menor ângulo de Norberg e maior grau de DCF. Pode-se concluir que o ângulo de inclinação e o índice cortical não demonstraram associação com a DCF.

A total of 386 radiographs of the pelvis were evaluated, being 220 of German Shepherd dogs (112 males and 108 females) and 166 of Labrador Retrievers (69 males and 97 females). The radiographs were degree classified regarding the of hip dysplasia (DHD). The Norberg and inclination angles, the cortico-medullary index, and the percentage coverage of the femoral head were measured and associated using multivariate statistical technique (principal component analysis). The cortico-medullary index and the inclination angle were inversely associated. The results indicated that German Shepherd Dogs showed lower cortico-medullary index and DHD compared with Labrador Retrievers. The higher the inclination angle, the lower the Norberg angle and percentage coverage of the femoral head. It was observed that with age, animals showed lower Norberg angles and percentage coverage of the femoral head, thus more hip dysplasia severity. Females were associated to lower femoral head coverage and Norberg angles, and more severe DHD. Femoral inclination angle and cortico-medullary index did not demonstrate a correlation to DHD.

Produção e qualidade de botões de rosa c v. Vega cultivados sob plásticos de cobertura com diferentes anos de uso / Production and quality of floral buds for rose cv. Vega (Rosa SP.) cultivated under plastic with different years of use

Objetivou-se, neste trabalho, avaliar a produção e qualidade de botões de rosa cultivar Vega ( Rosa sp. ), cultivada sob cobertura plástica com diferentes anos de uso. Para tanto foram selecionados 15 vãos de estufas com plásticos colocados em 1998, 1999, 2002, 2003, e testemunha (sem uso anterior), com três repetições, avaliados por duas maneiras: na primeira os frutos foram colhidos através de uma poda (como procedimento do cultivo comercial); na segunda foi realizada uma poda rasa em uma área de 1 m² para poder acompanhar o crescimento da haste floral desde o início da formação até a fase de botão floral. No primeiro caso foram realizadas 4 coletas ao longo do período do experimento, nas quais foram coletadas 4 hastes florais por tratamento, totalizando 60 flores por data de coleta. No segundo caso foi avaliado o aparecimento dos botões florais dos novos brotos em quatro datas até a formação das hastes florais comerciais, e então coletadas 13 hastes florais por tratamento. Não houve diferença estatística entre os anos de uso dos plásticos de 1998, 1999, 2002 e 2003 nas variáveis: comprimento, diâmetro e comprimento dos botões florais, massa fresca e seca das pétalas, para as condições e cultivar analisados. Com isso o produtor ganha um período maior de utilização do plástico gerando um custo menor, sem perdas na produção.

The aim of this study was to evaluate the production and quality of floral buds of rose cv. Vega (Rosa sp.) cultivated underneath a plastic film used as greenhouse covering, with different years of use. In order to evaluate the bud production and quality, 15 greenhouse gaps with plastic covering dating from 1998, 1999, 2002, 2003, were selected; and the control covering (never used before) with three replications each. Two types of evaluation were made for this analysis: the first one, the fruits were harvested by means of pruning (as the commercial use standard procedure); in the second evaluation a clear cut pruning was conducted in an area of 1m² with the purpose of determining the development of the flower buds from the pruning to the commercial flower stalk. On the first evaluation, 4 harvestings were conducted during the entire experiment period, in which 4 flower stalks per treatments were collected, totaling 60 flowers per collecting date. On the second evaluation, floral buds emerging on new shoots were determined in four different dates until commercial flower stalks complete formation was achieved, then 13 flower stalks per treatment were collected. There was no statistical difference among the plastic films with different years of use regarding stalk length and diameter, flower bud diameter and length, dry and fresh petal mass under the analyzed conditions and cultivars, translating into a larger period of plastic covering use without loss in the production at a low production cost.